Identification of Differentially Expressed Genes during Lace Plant Leaf Development

Programmed cell death remodels lace plant leaf shape during development.

Programmed cell death (PCD) functions in the developmental remodeling of leaf shape in higher plants, a process analogous to digit formation in the vertebrate limb. In this study, we provide a cytological characterization of the time course of events as PCD remodels young expanding leaves of the lace plant. Tonoplast rupture is the first PCD event in this system, indicated by alterations in cyt...

Identification of novel genes expressed in Brassica napus during leaf senescence and in response to oxidative stress

Senescence is a genetically regulated oxidative process that involves a general degradation of cellular structures and enzymes and the mobilization of the products of degradation to other parts of the plant. The cDNA-AFLP (cDNA-Amplified Fragment Length Polymorphism) analysis has been used under stringent PCR conditions afforded by ligation of adapters to restriction fragments, and the use of s...

identification of differentially expressed genes in l02 human hepatocyte induced by hepatitis c virus core protein

Identification of differentially expressed genes using minute amounts of RNA.

Identification of differentially expressed genes is critical to understand the molecular mechanisms underlying both normal and pathophysiological events. A significant limiting factor associated with such studies can often be the quantity of available starting material (i.e., RNA). Among the existing subtraction hybridization techniques that have been previously used for such studies at the lev...

Identification of differentially expressed genes in carp rods and cones

PURPOSE Rods and cones differ in their photoresponse characteristics, morphology, and susceptibilities to certain diseases. To contribute to the studies at the molecular level of these differences, we tried to identify genes expressed preferentially in rods or cones. METHODS From purified carp rods and cones, we extracted their RNA and obtained corresponding cDNA pools (rod cDNA and cone cDNA...